UCVMRESEARCH
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University of Calgary · Veterinary Medicine

This week in research.

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10Publications
07UCVM authors
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Here are the new works dated 2026-08-17 to 2026-08-24, with up to three concise points each and a final By the numbers summary. 1) Use of machine learning to detect Escherichia coli in drinking water in Bangladesh — PLoS ONE — 2026-08-20 — Cohort authors: Diego B. Nóbrega - Notable for comparing nine classical ML models plus a DL-MLP to predict E. coli contamination under imbalanced vs balanced data, with SHAP-based feature selection and SMOTE handling class imbalance. - It reports E. coli contamination in 39.2% of households (Bandarban district as a hotspot) and demonstrates more consistent model performance across algorithms after SMOTE and 10-fold cross-validation. - AdaBoost narrowly outperformed others in final evaluation, highlighting its robustness for this public health inference task. 2) Supplemental Materials: Qualitative Behavior Assessment reveals associations between affective states and rearing conditions of non-replacement dairy calves under commercial conditions — Figshare — 2026-08-20 — Cohort authors: Maria Camila Ceballos - Demonstrates Qualitative Behavioral Assessment as a welfare tool to measure emotional states of non-replacement dairy calves across commercial settings. - The material provides farm-level average scores for each behavioral descriptor from 19 calf-raising farms in the Midwest United States. - Emphasizes the feasibility of integrating affective-state metrics into routine welfare assessments. 3) Supplemental Materials: Qualitative Behavior Assessment reveals associations between affective states and rearing conditions of non-replacement dairy calves on commercial farms — Figshare — 2026-08-20 — Cohort authors: Maria Camila Ceballos - Reinforces the same welfare- and emotion-focused framework as the companion version, with emphasis on rearing-condition contrasts across commercial-farm contexts. - Adds dataset details or descriptors supporting cross-farm comparisons of affective-state indicators. - Highlights open-access supplemental data to accompany themain Qualitative Behavioral Assessment work. 4) Modulation of the nociceptive withdrawal reflex in walking is not changed by the presence of tonic pain — Experimental Brain Research — 2026-08-20 — Cohort authors: Jennifer Davies - Shows that tonic pain reduces contralateral tibialis anterior reflex amplitude during walking, but overall gait-phase–dependent modulation of the NWR remains largely unchanged. - Involves 16 healthy adults with EMG recordings from leg muscles during treadmill walking under pain vs control conditions. - Suggests that stabilizing factors for gait balance may override tonic pain effects on reflex modulation. 5) Method: How to investigate knowledge gaps and interrelationships between animal welfare and sustainability of animal production systems? — Zenodo (CERN) — 2026-08-20 — Cohort authors: Maria Camila Ceballos - Proposes a framework for mapping knowledge gaps and interrelationships between animal welfare and sustainability in production systems. - Provides a methodological approach for integrating welfare science with sustainability considerations, hosted openly on Zenodo. - Aims to guide researchers in designing studies that bridge welfare and sustainability disciplines. 6) Emergence of alveolar echinococcosis in Canada: a warning for the USA — Current Opinion in Infectious Diseases — 2026-08-20 — Cohort authors: Jamie L. Rothenburger - Documents a meaningful rise of locally acquired alveolar echinococcosis in Canada since 2013, signaling North American epidemiologic shifts. - Highlights factors such as the European-like Echinococcus multilocularis haplotype in wildlife and peri-urban dog exposure, plus heightened clinician awareness. - Advocates a One Health approach incorporating human, veterinary, and wildlife data to improve diagnosis, management, and outcomes. 7) Pre-existing Th1 immunity is abrogated by ongoing recruitment of monocytic host cells that are refractory to activation — bioRxiv (Cold Spring Harbor Laboratory) — 2026-08-17 — Cohort authors: Nathan C. Peters - In Leishmania major–model infection, pre-existing Th1 immunity is initially protective but is ultimately undermined by inflammatory monocyte–derived PD-L1+ PD-L2+ macrophages that create an intracellular niche. - Anti-PD-1 blockade enhances activation of uninfected monocyte-derived cells; depleting monocyte-derived precursors improves parasite control. - Concludes that evasion of pre-existing Th1 immunity stems from infected-cell–intrinsic activation defects rather than a failed Th1 response. 8) Incisor pathology in wild muskoxen (Ovibos moschatus) from the Canadian Arctic — Veterinary Pathology — 2026-08-17 — Cohort authors: Jamie L. Rothenburger; Douglas P. Whiteside; Susan Kutz - Reveals a high prevalence of incisor lesions in wild muskoxen, with enamel infractions in 91.4% (222/243) and complicated crown fractures in 50.6% (123/243) of individuals. - Additional radiographic findings include root fractures, hypercementosis, ankylosis, and other dental anomalies correlating with gross lesions. - Establishes foundational baseline data for muskox health and guides future epidemiological and welfare assessments in wild Arctic mammals. By the numbers - PLoS ONE: 1 - Figshare: 3 - Experimental Brain Research: 1 - Zenodo: 2 - Current Opinion in Infectious Diseases: 1 - bioRxiv: 1 - Veterinary Pathology: 1 Total works (2026-08-17 to 2026-08-24): 10

A closer look

Publications this week

10
PLoS ONE

Use of machine learning to detect Escherichia coli in drinking water in Bangladesh · doi/link

UCVM authors Diego B. Nóbrega

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Escherichia coli (E. coli) is a key indicator of fecal contamination in freshwater and can signal the presence of other harmful bacteria and viruses. The aim of the study is to evaluate the performance of machine learning (ML) tools to detect E. coli in drinking water in Bangladesh using surveillance data under two scenarios: an imbalanced dataset and a balanced dataset. We utilized data from the 2019 Bangladesh Multiple Indicator Cluster Survey, which included a total of 6,069 household drinking water samples. We used agglomerative hierarchical clustering with Ward's linkage to identify district-level hotspots. Extreme Gradient Boosting with SHapley Additive exPlanations values were used for feature selection, and the Synthetic Minority Over-sampling Technique (SMOTE) was used to address class imbalance in the classification task. We applied nine classical ML models in this study: Adaptive Boosting (AdaBoost), Decision Trees (DT), Gradient Boosting Algorithm (GBA), k-Nearest Neighbors (KNN), Light Gradient-Boosting Machine (LightGBM), Logistic Regression (LR), Naïve Bayes (NB), Random Forest (RF), and Support Vector Machine (SVM), along with a Deep Learning Multi-Layer Perceptron (DL-MLP) model to predict the risk of E. coli contamination (REcC) in water. Model performance was evaluated using accuracy, precision, recall, F1 score, Cohen Kappa, area under the curve (AUC), and a violin plot. E. coli contamination in drinking water was detected in 39.2% (95% CI: 37.4-41.2) of households. Bandarban district had the highest REcC. After applying SMOTE and 10-fold cross-validation with hyperparameter tuning, model performance was more consistent across algorithms. In terms of model evaluation, AdaBoost slightly outperformed the others with an accuracy of 81.6%, Cohen kappa statistic of 19.4%, precision of 82.2%, recall of 99%, F1-score of 89.8%, and an AUC of 68.6%. Ensemble model for example AdaBoost and GBA models had ability to accurately classify drinking water samples with respect to the presence of E. coli using surveillance data than others selected model in this study.
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Figshare

Supplemental Materials: Qualitative Behavior Assessment reveals associations between affective states and rearing conditions of non-replacement dairy calves under commercial conditions · doi/link

UCVM authors Maria Camila Ceballos

Read abstract
Qualitative Behavioral Assessment is a tool to measure emotional expression, an important component of animal welfare, that was implemented in this study to capture emotions of non-replacement dairy calves at calf raisers across the Midwestern United States. This supplemental file contains the average score for each behavioral descriptor by farm from Qualitative Behavioral Assessment performed on 19 calf-raising farms in the Midwestern United States.
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Figshare

Supplemental Materials: Qualitative Behavior Assessment reveals associations between affective states and rearing conditions of non-replacement dairy calves on commercial farms · doi/link

UCVM authors Maria Camila Ceballos

Read abstract
Qualitative Behavioral Assessment is a tool to measure emotional expression, an important component of animal welfare, that was implemented in this study to capture emotions of non-replacement dairy calves at calf raisers across the Midwestern United States. This supplemental file contains the average score for each behavioral descriptor by farm from Qualitative Behavioral Assessment performed on 19 calf-raising farms in the Midwestern United States.
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Figshare

Supplemental Materials: Qualitative Behavior Assessment reveals associations between affective states and rearing conditions of non-replacement dairy calves on commercial farms · doi/link

UCVM authors Maria Camila Ceballos

Read abstract
Qualitative Behavioral Assessment is a tool to measure emotional expression, an important component of animal welfare, that was implemented in this study to capture emotions of non-replacement dairy calves at calf raisers across the Midwestern United States. This supplemental file contains the average score for each behavioral descriptor by farm from Qualitative Behavioral Assessment performed on 19 calf-raising farms in the Midwestern United States.
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Experimental Brain Research

Modulation of the nociceptive withdrawal reflex in walking is not changed by the presence of tonic pain · doi/link

UCVM authors Jennifer Davies

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Tonic pain inhibits the nociceptive withdrawal reflex (NWR). NWR magnitude varies across the gait cycle, but it is unknown whether tonic pain affects this phase-dependent modulation of the NWR during walking. Sixteen healthy participants (mean ± SD age: 29 ± 9 years, 3 males) walked on a treadmill with and without tonic pain applied to the left arm. Lower-limb kinematics and electromyography (EMG) from bilateral biceps femoris, vastus lateralis, soleus and tibialis anterior were recorded. At predefined points in the gait cycle, a brief electrical stimulus was applied to the arch of the right foot to elicit an NWR. Reflex responses were calculated from the EMG as a percentage difference to the EMG present without any stimulation. There was a significant effect of gait phase on reflex response in all muscles except ipsilateral biceps femoris. When accounting for the intensity of tonic pain, there was a 0.64 decrease in normalised reflex response in the contralateral tibialis anterior with tonic pain versus control (p = 0.009). There were no other effects of tonic pain on reflex response (p > 0.05). There was an increase in knee flexion on the stimulated leg when it was unloading, however there was large variability in the kinematics. Stimulation during loading response saw around a 3° increase (p = 0.007) in dorsiflexion in the contralateral leg. Our findings suggest that tonic pain does not influence the modulation of the NWR during walking. We propose that the need to maintain balance overrides any effects of endogenous pain modulation.
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Current Opinion in Infectious Diseases

Emergence of alveolar echinococcosis in Canada: a warning for the USA · doi/link

UCVM authors Jamie L. Rothenburger

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PURPOSE OF REVIEW: Locally acquired human alveolar echinococcosis has been rare in North America outside Alaska, yet recent Canadian observations indicate a meaningful increase and epidemiologic shift in North America. This review synthesizes data surrounding the recent spike in human alveolar echinococcosis cases in Canada. RECENT FINDINGS: Since 2013, an increasing number of alveolar echinococcosis cases have been identified in Canada with incidentally detected hepatic masses. Dog ownership and rural exposures feature prominently. Contributory factors include the new establishment of the European-like Echinococcus multilocularis (Em) haplotype in wildlife and domestic dogs (definitive hosts), high peri-urban coyote numbers and infection prevalence, and rising clinician awareness. Specific diagnosis relies primarily on histopathology, confirmed with serology and Nucleic Acid Amplification Test (NAAT). Heightened awareness amongst radiologists and pathologists plays a pivotal role in improving patient outcomes. Management includes multidisciplinary collaborations involving resection when feasible and long-term albendazole treatment. SUMMARY: Alveolar echinococcosis in Canada is an emerging infectious zoonotic disease and remains under-recognized. A high index of suspicion is required, particularly for patients thought to have liver cancer. Early recognition combined with Em-specific diagnostics leads to timely treatment that reduces morbidity and mortality. A One Health approach integrating human, veterinary, and wildlife health data is needed to improve understanding of this disease.
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bioRxiv (Cold Spring Harbor Laboratory)

Pre-existing Th1 immunity is abrogated by ongoing recruitment of monocytic host cells that are refractory to activation · doi/link

UCVM authors Nathan C. Peters

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Abstract Protective immunity against many infectious diseases develops following primary infection, called infection induced immunity (III), and provides a blueprint for vaccination. However, many vaccination strategies have failed. In the parasitic Leishmania major model of self-healing cutaneous disease, control of secondary challenge infection relies on pre-existing T helper (Th)1-dependent activation of skin-infiltrating monocytes for elimination of intracellular parasites. To better understand immune-evasion of pre-existing Th1 immunity by pathogens, we investigated the pathogen-niche established following non-healing challenge infection with the L. amazonensis parasite in a setting of pre-existing III. Following secondary challenge, pre-existing Th1 III initially controlled infection but ultimately failed. Loss of protection was not overtly STAT6- or IL-10-mediated. Rather, monocyte-lineage tracing revealed inflammatory monocyte-derived PD-L1 + PD-L2 + macrophages provide an intracellular pathogen-niche and facilitate evasion of pre-existing Th1 immunity. Anti-PD-1 immune checkpoint blockade enhanced uninfected, but not infected, monocyte-derived cell activation and depletion of monocyte-derived precursors improved parasite control. These observations suggest that evasion of pre-existing Th1 immunity in this setting is not due to a failure of the Th1 response, but rather due to infected-cell intrinsic defects in activation.
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Veterinary Pathology

Incisor pathology in wild muskoxen ( Ovibos moschatus ) from the Canadian Arctic · doi/link

UCVM authors Jamie L. Rothenburger, Douglas P. Whiteside, Susan Kutz

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Dental lesions are often painful and can impair digestive efficiency and fitness in mammals, consequently leading to systemic health complications. Although dental lesions are rarely described in wild herbivores, a high prevalence of incisor abnormalities was detected in wild muskoxen ( Ovibos moschatus ) using mandibles collected by Inuit hunters through the Community-Based Wildlife Health Surveillance program in the Canadian Arctic. Here, we present the first comprehensive examination of incisor dental lesions and developmental anomalies in wild muskoxen. We examined 243 mandibles from harvested muskoxen and identified and described both gross and radiographic pathology. The most frequent lesion was enamel infractions, characterized by linear cracks along lingual enamel surfaces, which was present in 91.4% (222/243) of individuals. Incisor fractures, which featured at least one incisor with a complicated crown fracture, were present in 50.6% (123/243) of individuals. Labial enamel abrasions were present in 26.3% (64/243) of muskoxen in which wear extended to the dentin, and in severe cases to the pulp. Other common gross abnormalities included rotation (31.3%; 76/243), crowding (21.8%; 53/243), and unerupted fourth incisors (18.9%; 46/243). Radiographic analysis revealed congenitally absent teeth, root fractures, and endodontic-related lesions that correlated with gross incisor abnormalities in a subset of 136 animals. Other radiographic findings included hypercementosis, ankylosis, dilaceration, dental decay, and retained primary teeth. These results underscore the high prevalence and severity of incisor pathology in muskoxen and provide foundational data for subsequent assessment of lesion epidemiology and their association with muskox health.
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